Part:BBa_K1799015

pYeaR

Nitrate sensitive promoter

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

MIT_MAHE 2020

Summary

This is the promoter of the Escherichia coli yeaR/yoaG operon; responsive to nitrate, nitrite and nitric oxide.This is regulated mainly by phospho-NarL, although phospho-NarP can also activate it if NarL is not present. Repression of the promoter in the absence of nitrate/nitrite is mainly due to the repressor NsrR. Induction is higher under anaerobic conditions than under aerobic conditions, but strong induction still occurs under fully aerobic conditions. Unlike other E. coli promoters responding to nitrate and nitrite, this promoter is not repressed under aerobic conditions.

References

1. Lin, H. Y., Bledsoe, P. J., & Stewart, V. (2007). Activation of yeaR-yoaG operon transcription by the nitrate-responsive regulator NarL is independent of oxygen- responsive regulator Fnr in Escherichia coli K-12. Journal of bacteriology, 189(21), 7539–7548. https://doi.org/10.1128/JB.00953-07

2. Merulla, D., & van der Meer, J. R. (2016). Regulatable and Modulable Background Expression Control in Prokaryotic Synthetic Circuits by Auxiliary Repressor Binding Sites. ACS synthetic biology, 5(1), 36–45. https://doi.org/10.1021/acssynbio.5b00112